Oligo pools refer to large collections of synthetic oligonucleotides that are chemically synthesized in parallel. As described by Dynegene, a leading company in ultra-high-throughput DNA synthesis, oligo pools provide a foundation for constructing diverse DNA libraries utilized in cutting-edge applications across synthetic biology, molecular diagnostics, and biomedical research.

Key Characteristics of Oligo Pools

Oligo pools contain thousands to millions of distinct oligo sequences in a single low-cost reagent. The oligos are generally 20-350 nucleotide bases long and can be synthesized at very high scales, with 10-100X lower costs compared to conventional column-based DNA synthesis. However, the individual oligos exist at very low concentrations within the complex pools. The synthesis process also leads to uneven representation of sequences and higher error rates.

Advances in Oligo Pool Technology

To address these limitations, novel techniques have been developed by companies like Dynegene to enhance the quality and utility of oligo pools. These include:

• Proprietary DYHOW Technology-based synthesis chemistry for improved accuracy
• Molecular barcodes and NGS-based QC to verify sequences
• Advanced pool designs and amplification methods to normalize representation

Applications of Oligo Pools

With recent advances, oligo pools now enable scalable and cost-effective construction of complex DNA libraries for cutting-edge applications:

High-Throughput Functional Genomics

• CRISPR guide RNA libraries for genome-wide genetic screens
• DNA barcode libraries for spatial transcriptomics

Targeted Sequencing

• Hybridization probes for exome capture and enrichment

Protein Engineering

• Mutant DNA libraries for directed evolution
• Synthetic gene assembly from scratch

Emerging Technologies

• DNA data storage
• In situ sequencing assays like oligoFISH

So in summary, oligo pools now provide a scalable, low-cost foundation for constructing complex next-generation DNA libraries that are driving innovation across genomics, molecular diagnostics, biotechnology, and even data storage.

Conclusion

Dynegene is at the forefront of commercializing these emerging ultra-high-throughput DNA synthesis technologies in China. Their proprietary approaches for enhancing oligo pool quality are helping to overcome limitations and unlock the potential of oligo pools for advanced applications from functional genomics screens to synthetic genome construction.